This paper presents the results of an acoustic study designed to determine whether or not ATR (Advanced Tongue Root) features which are contrastive in Kinande high vowels are also contrastive in high glides derived from them. F1 and F2 values, the primary acoustic correlates distinguishing [+ATR] high vowels from [-ATR] high vowels in Kinande (Gick et. al 2006, Kenstowicz 2009), were obtained for glides derived from [-ATR] [i] and [+ATR] [ị] in comparable segmental and prosodic contexts. Glides derived from the [+ATR] vowels were observed to have significantly lower F1 values and significantly higher F2 values than glides derived from the [-ATR] vowels, suggesting that the ATR contrast is preserved under glide formation.